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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha : |
21/02/2014 |
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Actualizado : |
06/06/2022 |
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Autor : |
KOHLI, M.M.; FRANCIS, M. (Ed.). |
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Afiliación : |
MAN MOHAN KOHLI; MARTHA FRANCIS. |
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Título : |
Application of biotechnologies to wheat breeding. Proceedings of a conference at La Estanzuela, Uruguay. November 19-20, 1998. |
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Fecha de publicación : |
2000 |
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Fuente / Imprenta : |
Montevideo (Uruguay): Instituto Nacional de Investigación Agropecuaria; Centro Internacional de Mejoramiento de Maíz y Trigo, 2000. |
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Páginas : |
175 p. |
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ISBN : |
9974-7586-1-0 |
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Idioma : |
Español
Inglés |
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Contenido : |
The application of biotechn'ology to crop improvement has been the focus of much research around the world. Tissue culture and selection of somaclonal variation were developed many years ago, followed more recently by molecular markers and genetic engineering. This meeting focused on providing updates on the actual successes to date on the application of several areas of biotechnology to wheat improvement. What is clear is that there continues to be optimism regarding the potential for biotechnology to be applied to wheat improvement. It is also noteworthy that as was predicted by many scientists, many approaches are proving more difficult in wheat than in other crops. Molecular markers, for example, are difficult to apply routinely given the large genome, hexaploid genetics, and lower levels of polymorphisms. New marker systems such as AFLPs and microsatellites are an improvement over previous RFLPs, but still require significant investments to achieve similar results possible in other, more amenable species. What is encouraging are the successes to identify the genetics of complex traits in wheat such as quality, Fusarium and rust resistance. Some of these will surely provide useful applications in wheat improvement and all mapping efforts provide critical genetic information for further studies at the gene level. These will be increasingly important as genomic approaches (gene expression, proteomics, etc.) become available and are applied to wheat. In many ways, these latest genomics approaches should be more 'genome independent' and thus readily applicable to large, complex genome species such as wheat. Coupled with the relatively easy ability to produce doubled haploids, wheat may prove to be an extremely important species for genomic studies. MenosThe application of biotechn'ology to crop improvement has been the focus of much research around the world. Tissue culture and selection of somaclonal variation were developed many years ago, followed more recently by molecular markers and genetic engineering. This meeting focused on providing updates on the actual successes to date on the application of several areas of biotechnology to wheat improvement. What is clear is that there continues to be optimism regarding the potential for biotechnology to be applied to wheat improvement. It is also noteworthy that as was predicted by many scientists, many approaches are proving more difficult in wheat than in other crops. Molecular markers, for example, are difficult to apply routinely given the large genome, hexaploid genetics, and lower levels of polymorphisms. New marker systems such as AFLPs and microsatellites are an improvement over previous RFLPs, but still require significant investments to achieve similar results possible in other, more amenable species. What is encouraging are the successes to identify the genetics of complex traits in wheat such as quality, Fusarium and rust resistance. Some of these will surely provide useful applications in wheat improvement and all mapping efforts provide critical genetic information for further studies at the gene level. These will be increasingly important as genomic approaches (gene expression, proteomics, etc.) become available and are applied to wheat. In many ways, these lat... Presentar Todo |
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Palabras claves : |
BIOTECHNOLOGY; GENETIC TRANSFORMATION; MOLECULAR MARKERS; PLANT BREEDING; RESEARCH PROYECTS; TRITICUM AESTIVUM. |
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Thesagro : |
ARGENTINA; BIOTECNOLOGIA; BRASIL; FITOMEJORAMIENTO; MARCADORES MOLECULARES; MEXICO; PROYECTOS DE INVESTIGACION; SUD AMERICA; TRANSFORMACION GENETICA; TRIGO; TRITICUM AESTIVUM; URUGUAY; USA. |
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Asunto categoría : |
--
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5843/1/RBI-5022-Application.pdf
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Marc : |
LEADER 02905nam a2200373 a 4500
001 1055271
005 2022-06-06
008 2000 bl uuuu u00u1 u #d
020 $a9974-7586-1-0
100 1 $aKOHLI, M.M.
245 $aApplication of biotechnologies to wheat breeding. Proceedings of a conference at La Estanzuela, Uruguay. November 19-20, 1998.
260 $aMontevideo (Uruguay): Instituto Nacional de Investigación Agropecuaria; Centro Internacional de Mejoramiento de Maíz y Trigo$c2000
300 $a175 p.
520 $aThe application of biotechn'ology to crop improvement has been the focus of much research around the world. Tissue culture and selection of somaclonal variation were developed many years ago, followed more recently by molecular markers and genetic engineering. This meeting focused on providing updates on the actual successes to date on the application of several areas of biotechnology to wheat improvement. What is clear is that there continues to be optimism regarding the potential for biotechnology to be applied to wheat improvement. It is also noteworthy that as was predicted by many scientists, many approaches are proving more difficult in wheat than in other crops. Molecular markers, for example, are difficult to apply routinely given the large genome, hexaploid genetics, and lower levels of polymorphisms. New marker systems such as AFLPs and microsatellites are an improvement over previous RFLPs, but still require significant investments to achieve similar results possible in other, more amenable species. What is encouraging are the successes to identify the genetics of complex traits in wheat such as quality, Fusarium and rust resistance. Some of these will surely provide useful applications in wheat improvement and all mapping efforts provide critical genetic information for further studies at the gene level. These will be increasingly important as genomic approaches (gene expression, proteomics, etc.) become available and are applied to wheat. In many ways, these latest genomics approaches should be more 'genome independent' and thus readily applicable to large, complex genome species such as wheat. Coupled with the relatively easy ability to produce doubled haploids, wheat may prove to be an extremely important species for genomic studies.
650 $aARGENTINA
650 $aBIOTECNOLOGIA
650 $aBRASIL
650 $aFITOMEJORAMIENTO
650 $aMARCADORES MOLECULARES
650 $aMEXICO
650 $aPROYECTOS DE INVESTIGACION
650 $aSUD AMERICA
650 $aTRANSFORMACION GENETICA
650 $aTRIGO
650 $aTRITICUM AESTIVUM
650 $aURUGUAY
650 $aUSA
653 $aBIOTECHNOLOGY
653 $aGENETIC TRANSFORMATION
653 $aMOLECULAR MARKERS
653 $aPLANT BREEDING
653 $aRESEARCH PROYECTS
653 $aTRITICUM AESTIVUM
700 1 $aFRANCIS, M.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
24/01/2020 |
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Actualizado : |
10/02/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MACÍAS-RIOSECO, M.; CAFFARENA, D.; FRAGA, M.; SILVEIRA, C.S.; GIANNITTI, F.; CANTÓN, G.; HECKER, Y.P.; SUANES, A.; RIET-CORREA, F. |
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Afiliación : |
MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidad de la República, Facultad de Veterinaria, Montevideo, Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; University of Minnesota, Veterinary Population Medicine Department, MN, United States; GERMÁN CANTÓN, Instituto Nacional de Tecnología Agropecuaria, Balcarce, Argentina; YANINA P. HECKER, Consejo Nacional de Investigaciones Científicas y Técnicas, Balcarce, Argentina; ALEJANDRA SUANES, Ministerio de Ganadería Agricultura y Pesca, Montevideo. Dirección de Laboratorios Veterinarios, Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd. (Technical note). [Brote de abortos causado por Campylobacter fetus subespecie venerealis y Neospora caninum en un hato bovino lechero. (Nota de investigación)]. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Revista Mexicana De Ciencias Pecuarias, 2019, Volume 10, Issue 4, 2019, Pages 1054-1063. OPEN ACCESS. Doi: 10.22319/rmcp.v10i4.5008 |
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ISSN : |
2007-1124 |
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DOI : |
10.22319/rmcp.v10i4.5008 |
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Idioma : |
Inglés |
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Notas : |
Article history: Recibido: 02 Agosto 2018. / Aceptado: 28 Agosto 2018. |
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Contenido : |
ABSTRACT.
In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous epicarditis and peritonitis, and neutrophilic bronchopneumonia. Campylobacter fetus subsp. venerealis was detected by direct immunofluorescence, isolated and identified by PCR and sequencing of the 16S rDNA in the abomasal fluid and/or lung. Histologic examination of two other fetuses revealed non-suppurative necrotizing encephalitis, lymphohistiocytic myositis and myocarditis, and lymphocytic interstitial nephritis. In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. Antibodies against N. caninum were detected by indirect immunofluorescence in 10 of 27 cows, with titers ranging from 1/200 to 1/3200. The results indicate that two abortigenic microorganisms may coexist and cause contemporaneous abortion in a herd. It is relevant to highlight the importance of performing multiple diagnostic tests in various aborted dams and fetuses from the same herd for the etiologic confirmation of bovine abortion syndrome.
© 2019 INIFAP-CENID Parasitologia Veterinaria. All rights reserved.
RESUMEN.
En noviembre de 2015 ocurrió un brote de abortos en un hato lechero comercial de 650 vacas Holstein en el departamento de Florida, Uruguay. Cuarenta y cinco (45) vacas abortaron en un lapso de 3 semanas. Cinco fetos fueron sometidos a un examen patológico macro y microscópico y a pruebas microbiológicas. Un feto tenía epicarditis fibrinosa y peritonitis, así como bronconeumonía neutrofílica. Se detectó Campylobacter fetus subesp. venerealis utilizando inmunofluorescencia directa; se lo aisló e identificó mediante PCR y secuenciación del 16S rDNA en el líquido abomasal y en el pulmón. El examen histológico de otros dos fetos reveló encefalitis necrotizante no supurativa, miositis linfohistiocítica y miocarditis, y nefritis linfocítica intersticial. En estos fetos se detectó intralesionalmente el antígeno de N. caninum mediante análisis inmunohistoquímico, y se amplificó el ADN de N. caninum mediante PCR en tejido cerebral fijado con formalina y embebido en parafina. Se detectaron anticuerpos contra N. caninum mediante inmunofluorescencia indirecta en 10 de 27 vacas, con títulos de entre 1/200 y 1/3200. Los resultados indican que dos microorganismos abortígenos pueden coexistir y provocar abortos contemporáneos en un hato. Subrayamos la importancia de realizar pruebas diagnósticas múltiples en diversas madres abortadas y fetos del mismo hato para obtener una confirmación etiológica del síndrome de aborto bovino. MenosABSTRACT.
In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous epicarditis and peritonitis, and neutrophilic bronchopneumonia. Campylobacter fetus subsp. venerealis was detected by direct immunofluorescence, isolated and identified by PCR and sequencing of the 16S rDNA in the abomasal fluid and/or lung. Histologic examination of two other fetuses revealed non-suppurative necrotizing encephalitis, lymphohistiocytic myositis and myocarditis, and lymphocytic interstitial nephritis. In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. Antibodies against N. caninum were detected by indirect immunofluorescence in 10 of 27 cows, with titers ranging from 1/200 to 1/3200. The results indicate that two abortigenic microorganisms may coexist and cause contemporaneous abortion in a herd. It is relevant to highlight the importance of performing multiple diagnostic tests in various aborted dams and fetuses from the same herd for the etiologic confirmation of bovine abortion syndrome.
© 2019 INIFAP-CENID Parasitologia Veterinaria. All rights reserved.
RESUMEN.
En noviembre de 2015 ocurrió un brote de abortos en un hato lechero... Presentar Todo |
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Palabras claves : |
Bovine abortion; Campylobacter fetus subsp. venerealis; Diagnosis of abortion; Neospora caninum; PLATAFORMA SALUD ANIMAL. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14066/1/5008-14394-1-PB.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/14067/1/5008-14395-1-PB.pdf
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Marc : |
LEADER 04147naa a2200313 a 4500
001 1060687
005 2020-02-10
008 2019 bl uuuu u00u1 u #d
022 $a2007-1124
024 7 $a10.22319/rmcp.v10i4.5008$2DOI
100 1 $aMACÍAS-RIOSECO, M.
245 $aAbortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd. (Technical note). [Brote de abortos causado por Campylobacter fetus subespecie venerealis y Neospora caninum en un hato bovino lechero. (Nota de investigación)].$h[electronic resource]
260 $c2019
500 $aArticle history: Recibido: 02 Agosto 2018. / Aceptado: 28 Agosto 2018.
520 $aABSTRACT. In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous epicarditis and peritonitis, and neutrophilic bronchopneumonia. Campylobacter fetus subsp. venerealis was detected by direct immunofluorescence, isolated and identified by PCR and sequencing of the 16S rDNA in the abomasal fluid and/or lung. Histologic examination of two other fetuses revealed non-suppurative necrotizing encephalitis, lymphohistiocytic myositis and myocarditis, and lymphocytic interstitial nephritis. In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. Antibodies against N. caninum were detected by indirect immunofluorescence in 10 of 27 cows, with titers ranging from 1/200 to 1/3200. The results indicate that two abortigenic microorganisms may coexist and cause contemporaneous abortion in a herd. It is relevant to highlight the importance of performing multiple diagnostic tests in various aborted dams and fetuses from the same herd for the etiologic confirmation of bovine abortion syndrome. © 2019 INIFAP-CENID Parasitologia Veterinaria. All rights reserved. RESUMEN. En noviembre de 2015 ocurrió un brote de abortos en un hato lechero comercial de 650 vacas Holstein en el departamento de Florida, Uruguay. Cuarenta y cinco (45) vacas abortaron en un lapso de 3 semanas. Cinco fetos fueron sometidos a un examen patológico macro y microscópico y a pruebas microbiológicas. Un feto tenía epicarditis fibrinosa y peritonitis, así como bronconeumonía neutrofílica. Se detectó Campylobacter fetus subesp. venerealis utilizando inmunofluorescencia directa; se lo aisló e identificó mediante PCR y secuenciación del 16S rDNA en el líquido abomasal y en el pulmón. El examen histológico de otros dos fetos reveló encefalitis necrotizante no supurativa, miositis linfohistiocítica y miocarditis, y nefritis linfocítica intersticial. En estos fetos se detectó intralesionalmente el antígeno de N. caninum mediante análisis inmunohistoquímico, y se amplificó el ADN de N. caninum mediante PCR en tejido cerebral fijado con formalina y embebido en parafina. Se detectaron anticuerpos contra N. caninum mediante inmunofluorescencia indirecta en 10 de 27 vacas, con títulos de entre 1/200 y 1/3200. Los resultados indican que dos microorganismos abortígenos pueden coexistir y provocar abortos contemporáneos en un hato. Subrayamos la importancia de realizar pruebas diagnósticas múltiples en diversas madres abortadas y fetos del mismo hato para obtener una confirmación etiológica del síndrome de aborto bovino.
653 $aBovine abortion
653 $aCampylobacter fetus subsp. venerealis
653 $aDiagnosis of abortion
653 $aNeospora caninum
653 $aPLATAFORMA SALUD ANIMAL
700 1 $aCAFFARENA, D.
700 1 $aFRAGA, M.
700 1 $aSILVEIRA, C.S.
700 1 $aGIANNITTI, F.
700 1 $aCANTÓN, G.
700 1 $aHECKER, Y.P.
700 1 $aSUANES, A.
700 1 $aRIET-CORREA, F.
773 $tRevista Mexicana De Ciencias Pecuarias, 2019, Volume 10, Issue 4, 2019, Pages 1054-1063. OPEN ACCESS. Doi: 10.22319/rmcp.v10i4.5008
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